During Phase 1, we will perform all five double digests listed in the Peterson (et. GBS protocol development service: There are two phases to this service. Two-step gene-specific PCR with dual indexes, bacterial 16S V4/V5 region (The GSAF can normalize your sample concentrations for an additional charge.) # of Samples See this web page for sample input guidelines and note that DNA concentrations should be normalized before submission. DNA is expected as input the GSAF does not provide DNA extraction services at this time. Gel-based QC is performed on a sampling of 10% of the samples post amplification. The prices listed below include sample prep from genomic DNA and sequencing. PCR is performed in triplicate to minimize jackpot effects. equal concentration) DNA samples and includes amplification of the V4/V5 regions of the bacterial 16S gene as shown on this web page and at least 10,000 2x250 bp paired-end sequences from the Illumina MiSeq platform for at least 95% of the samples submitted. Metagenomics assay: This service starts with normalized (i.e. Other ribosomal RNA removal kits may also be requested, but you may have to pay for an entire kit if it is unlikely to be used for other projects. *The GSAF stocks the Epicenter human/mouse/rat RiboZero rRNA removal kit the human/mouse/rat Gold, bacteria (gram-positive and -negative) are also commonly used but may not be in stock with the GSAF. RNA directional with index - NO removal of ribosomal RNA RNA directional WITH poly-A enrichment and index See this web page for sample input guidelines. Price includes barcoding/indexing and final QC by BioAnalyzer and qPCR. Always processed in batches of at least 8 if you submit fewer than 8 samples, your job will be queued until a minimum batch size of 8 is reached. RNA low cost high throughput - size range typically ~200 nt "dUTP" directional protocol. Low cost, high throughput DNA and RNA prepsĭNA low cost high throughput - wide size range, ~300 bp insert size. 16S rRNA)įor more info, please see the GSAF wiki on pricing. 16S rRNA)īacterial genome assembly, longer amplicons (e.g. Small RNA, RNA-seq, ChIP-seq, reference-guided assembly, DNA variantsĭe novo RNA & DNA sequencing, splice variant RNA-seq, DNA variantsĭe novo RNA & DNA sequencing, amplicon sequencing (e.g. Sequencing Sequencing (Illumina platform): price per lane is as follows.
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